fluorocell wdf fluorescent reagent  (Sysmex Corporation)

Journal: Cell

Article Title: Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment

doi: 10.1016/j.cell.2020.08.001

Figure Lengend Snippet:

Article Snippet: Fluorocell™ WDF , Sysmex , Cat# CV-377-552.

Techniques: Purification, Produced, Recombinant, Staining, Lysis, Blocking Assay, Labeling, Selection, Multiplexing, Amplification, Isolation, Software, Derivative Assay

Journal: PLoS ONE

Article Title: Novel flowcytometry-based approach of malignant cell detection in body fluids using an automated hematology analyzer

doi: 10.1371/journal.pone.0190886

Figure Lengend Snippet: (A) Area 1 includes the aggregated cells due to the high forward scatter width signals that implies the long “time of flight” of the cells passing though the laser beam in the flow-cell. The HF-BF cells gated in Area 1 are considered as the Rule1 positive cells. The horizontal axis indicates forward scatter pulse width signal and the vertical axis shows forward scatter signal in the 2D scattergram. In the 3D scattergram, X-axis shows side scatter signal, Y-axis is forward scatter signal and Z-axis indicates forward scatter pulse width signal. (B) Area 2 includes the non-aggregated HF-BF cell showing relatively low fluorescent signal intensity, which are defined as the Rule 2 positive cells. The horizontal axis indicates forward scatter signal and the vertical axis shows side fluorescence signal in the 2D scattergram. In the 3D scattergram, X-axis indicates side scatter signal, Y-axis shows side fluorescence signal and Z-axis is forward scatter signal. The gating illustrated based on the plotting WDF channel dot plots.

Article Snippet: To count and differentiate nucleated cells in BF, the cell membranes are perforated with Lysercell WDF TM (Sysmex, Kobe, Japan), then intracellular organelles and nucleic acids are stained with Fluorocell WDF TM (Sysmex, Kobe, Japan) so that the fluorescence flow cytometric analyses can detect specific side scattered signals which are generated depending on the type and quantity of intracellular organelles and nucleic acids.

Techniques: Fluorescence